Enhancing human memory through gene editing technology is a complex and challenging field that requires a combination of the latest advances in neuroscience, genetics, and biotechnology. The following is a framework of potential strategies and experimental steps based on current scientific research, but it should be noted that such research must strictly abide by ethical norms and has not yet entered the clinical application stage. --- ###**1. Potential target genes and mechanisms** 1. **CREB (cAMP response element binding protein)** -**Function**: Regulates synaptic plasticity and long-term memory formation, and promotes the enhancement of connections between neurons after activation. -**Editing strategy**: Enhance CREB gene expression or extend its activity through the CRISPR/dCas9 system. 2. **BDNF (brain-derived neurotrophic factor)** -**Function**: Promote neuronal survival, synaptic plasticity and memory consolidation. -**Editing strategy**: upregulate BDNF gene expression or optimize its Val66Met polymorphism. 3. **NR2B (NMDA receptor subunit)** -**Function**: Regulates the transmission of synaptic signals related to learning and memory. -**Editing strategy**: Extend the expression of NR2B in the adult brain through gene editing (similar to the “smart mouse” experiment). 4. **Epigenetic regulatory genes (such as HDAC2)** -**Effect**: Histone deacetylase HDAC2 inhibits the expression of memory-related genes, and knocking down HDAC2 can enhance memory. --- ###**2. Experimental steps (take a mouse model as an example)** ####**Stage 1: In vitro cell experiment** 1. **Target verification** -Screening candidate genes (such as CREB, BDNF) in neuronal cell lines, and regulating gene expression through CRISPR activation (CRISPRa) or inhibition (CRISPRi) techniques. -Detect changes in synaptic markers (such as PSD-95) and neurotransmitter release. 2. **Off-target effect assessment** -Verify editing specificity using whole genome sequencing (WGS) or targeted sequencing. ####**Stage 2: Animal model construction** 1. **Delivery of gene editing tools** -**Method**: The CRISPR system is delivered to the hippocampus (a key brain area for memory) of mice through AAV viral vectors or nano-particles. -**Control group **: Injection