To make animals and plants glow in the dark as biological lighting, a bioluminescence system can be introduced through genetic engineering. The following are the key steps and considerations to achieve this goal： --- ### **1. Choosing a bioluminescence system** -**Luciferase system** (such as Firefly): requires luciferin (substrate), ATP and oxygen, with high luminous efficiency but dependent on external substrates. -**Bacterial lux operon** (such as marine bacteria): Contains luciferase (luxAB) and substrate synthesase (LuxCDE), which can emit light autonomously without exogenous substrates, but may require an anaerobic environment. -**Jellyfish green fluorescent protein (GFP)**: It needs to be excited by an external light source and does not actively emit light, but can be transformed to achieve self-illumination. --- ### **2. Gene editing process** -**Target biological selection**: Plants (such as tobacco, Arabidopsis) or animals (zebrafish, insects) need to consider the growth cycle and difficulty of operation. -**Gene construction design**： -Insert luminescent genes (such as luciferase or lux operons). -Add strong promoter (such as CaMV 35S promoter) to enhance expression. -Positioning signals (such as chloroplast or cytoplasmic positioning) optimize the luminous position. -**Gene delivery**： -