# Experimental design of genetic modification to enhance human sensory abilities ## Research objectives Design a progressive study to explore the safe enhancement of human visual and auditory acuity and neural response speed through genetic modification technology. ## Ethical framework The experimental design must follow the following ethical principles： * Strictly abide by the international code of Ethics for human experiments * Approved by multiple ethics committees * Full informed consent of all participants * Adopt reversible or controllable technology * Establish an independent supervision mechanism ## Research stage ### The first stage: Animal model research 1. **Genetic screening and analysis** * Study genes known to enhance vision (such as the PDE6 gene related to night vision) *Analyze auditory-related genes (such as hair cell development-related gene ATOH1) *Study genes related to nerve conduction velocity (such as ion channel protein coding genes) 2. **Primate verification experiment** * Use CRISPR-Cas9 technology for gene editing * Long-term follow-up research to evaluate safety and functional changes ### The second stage: Human cell experiment 1. **In vitro human tissue culture** * Construct organoid culture of human retina and inner ear tissue * Test the effect of genetic modification on organoids 2. **Safety assessment** * Genome-wide off-target effect analysis * Immunogenicity research * Long-term stability assessment ###The third stage: preclinical research 1. **Develop delivery system** * Design AAV (adeno-associated virus) vectors for the eye and inner ear * Develop controllable expression systems (such as inductive promoter) 2. **Design safety mechanism** *Implement a "turn off switch" for gene expression * Design of reversible modification strategy ### The fourth stage: limited human trials (from the perspective of medical repair) 1. **Recruitment criteria** * First target patients with specific sensory impairments (such as night blindness, hearing loss) * Strict health screening standards 2. **Interventions** * Delivery of gene therapy to target tissues * Adopt the minimum effective dose strategy 3. **Monitoring and evaluation** * Comprehensive physical and psychological assessment *Objective measurement of visual/auditory function *Neural response speed test * Long-term follow-up plan (5-10 years) ## Technical route 1. **Visual enhancement strategy** * Rhodopsin gene optimization to enhance low-light vision * Regulation of cone cell density or function to improve color recognition * Optimization of ocular nerve conduction 2. **Auditory enhancement strategy** * Hair cell regeneration and function enhancement * Adjustment of cochlear sensitivity * Optimization of auditory processing neural pathways 3. **Enhanced response speed** * Optimize neurotransmitter release mechanism * Enhance synaptic formation and plasticity *Ion channel modification to increase conduction speed ##Risk Management 1. **Medical risks**: Excessive sensitivity leads to sensory overload, inflammatory response, and immune rejection 2