In theory, the current genetic modification technology cannot completely eliminate the human aging process, but scientists are conducting related research to explore its possibilities. The following is an example of a hypothetical experimental plan, but this is currently only based on scientific ideas and faces many ethical and technical obstacles in practice.： **Outline of experimental plan**： ###1. Experimental objectives Explore the regulation of specific genes through gene editing techniques to delay or improve physiological changes related to aging. ### 2. Experimental subjects Choose some healthy experimental animal models, such as mice, while considering the gradual transition to primates in the future. ###3. Gene editing strategy 1. Identify key genes related to aging, such as genes involved in processes such as cell aging, telomere maintenance, DNA repair, and metabolic regulation. 2. Use gene editing tools, such as the CRISPR/Cas9 system, to perform targeted editing of selected genes. 3. Design different editing schemes for different genes, including gene knockouts, gene activation, or gene modification, to observe the effects on aging-related phenotypes. ###4. Experimental grouping 1. **Wild-type control group**: Normal animals that have not been genetically edited are used as controls for comparison. 2. **Gene editing experimental groups**: Multiple experimental groups are set up according to different gene editing strategies, each group editing different genes or using different editing methods. ###5. Monitoring of experimental indicators 1. **Physiological indicators** -Regularly measure basic physiological parameters such as weight, body length, and proportion of various parts of the body. -Monitor cardiovascular and respiratory indicators such as heart rate, blood pressure, and respiratory rate. 2. **Cellular and molecular level indicators** -Extract blood and tissue samples to detect cell aging-related markers, such as aging-related β-galactosidase activity. - Analyze telomere length changes and detect telomerase activity by quantitative PCR and other methods. -Conduct transcriptome analysis, observe changes in gene expression profiles, and screen for expression differences in genes related to aging. -Detect oxidative stress-related indicators, such as reactive oxygen species (ROS) levels, antioxidant enzyme activity, etc. 3. **Tissue and organ function indicators** -Observe the structural changes of different tissues and organs, such as the liver, kidneys, muscles, brain, etc., through histopathological methods. -Evaluate muscle strength and athletic ability, such as grip strength test, running endurance test, etc. -Detect cognitive function and evaluate learning and memory ability through behavioral experiments, such as the Morris water maze experiment. ###6. Experimental steps 1. **Gene editing operations** -Introduce the constructed gene editing vector into the fertilized eggs or embryonic stem cells of experimental animals through appropriate methods. -Cells or embryos with successful gene editing are screened and transplanted into surrogate animals for development. 2. **Breeding and observation of experimental animals** -The laboratory animals after birth are raised in groups in a standard environment and given the same diet and living conditions. -Regularly monitor various indicators of experimental animals and record data. 3. **Long-term tracking** -Continuously observe the entire life cycle of experimental animals from infancy to old age, and compare the physiological changes and differences in aging-related indicators of different groups of animals at different ages. ###7. Data analysis Conduct statistical analysis of a large number of experimental data collected, compare the differences in various indicators between different groups, and evaluate the impact of gene editing on the aging process. It should be emphasized that conducting such experiments on humans faces extremely strict ethical scrutiny and legal restrictions. Moreover, there are still many unknowns about the relationship between genes and aging, and the goal of completely eliminating aging is still very far away.