# Experimental scheme for genetic modification to enhance human senses and reaction speed ##1. Purpose of experiment The purpose of this experiment is to explore the possibility of enhancing the acuity and reaction speed of human vision and hearing through genetic modification technology, and to provide a theoretical and practical basis for the improvement of human perception and physical function in the future. ## 2. Experimental subjects Volunteers who are between the ages of 20-35, are in good health, and do not have major genetic diseases and related sensory diseases are recruited. The number of recruits is expected to be [X] people. ##3. Experimental hypothesis Through the modification of specific genes, the development and function of vision and auditory-related neural pathways, as well as the response mechanism of the nervous system to external stimuli, can be regulated, thereby improving visual acuity, auditory acuity and response speed. ##4. Experimental design In this experiment, gene editing technology was used to genetically modify the subjects, and a strict control group was set up for comparative observation to evaluate the effect of genetic modification. ### Gene selection and editing strategy 1. **Vision-related genes**: Select genes related to retinal photoreceptor development and visual information transmission, such as Opsin genes. Through CRISPR/Cas9 gene editing technology, these genes are specifically modified to enhance the sensitivity of their encoded protein to light and the efficiency of signal transmission. 2. **Hearing-related genes**: Target genes related to the development of hair cells in the inner ear and auditory nerve conduction, such as the Myosin VIIa gene (Myosin VIIa gene). Gene editing technology is used to adjust its expression level or function to improve the ability to capture and transmit auditory signals. 3. **Response speed-related genes**: Study genes related to synaptic plasticity and regulation of neuronal excitability, such as Potassium channel genes. Through genetic modification, the conduction speed of nerve signals and the efficiency of information processing between neurons are optimized, thereby improving the response speed. ### Experiment group 1. **Experimental group**: Genetic modification operations were performed on [X/2] volunteers, and genes related to vision, hearing, and response speed were edited in accordance with the above gene selection and editing strategies. 2. **Control group**: In addition, [X/2] volunteers were selected as controls. No genetic modification operations were carried out, but the same living environment, diet and daily activity arrangements were given to the experimental group to exclude interference from other factors. Experimental results. ### Experimental process 1. **Pre-experiment preparation stage (week 1-2)** -Conduct a comprehensive physical examination of all volunteers, including vision, hearing, and reaction speed tests (such as visual response, auditory response, etc.), as well as detailed genetic testing to determine their initial status. -Train volunteers to familiarize them with various operations and test methods during the experiment to ensure the accuracy and reliability of the experimental data. 2. **Genetic modification stage (week 3-4)** - For volunteers in the experimental group, CRISPR/Cas9 gene editing technology was used to modify the target gene under strict laboratory conditions. The physical response of volunteers is closely monitored during the transformation process to ensure the safety of operation. -After the transformation is completed, the volunteers in the experimental group will be genetically sequenced to verify whether the gene editing is successful and whether the expected transformation effect is achieved. 3. **Observation and monitoring phase (weeks 5-16)** -Regularly (once a week) test the vision, hearing and reaction speed of the volunteers in the experimental group and the control group to record the changes in various indicators. -Observe the volunteers' visual and auditory performance in their daily lives, such as their perception of subtle visual stimuli, their ability to distinguish weak sounds, etc., and subjectively evaluate and record them. - In the 8th and 16th weeks, the volunteers were given a comprehensive physical examination to assess whether genetic modification has a potential impact on other physiological functions. 4. **Data analysis and Result evaluation stage (Week 17-18)** -Perform a statistical analysis of all the collected experimental data, compare the differences between the experimental group and the control group in terms of visual and auditory acuity and reaction speed, and evaluate the effect of genetic modification. -Comprehensively consider the observation indicators and physical examination results during the experiment, and analyze whether there are potential risks or adverse effects of genetic modification. ##5. Expected results of the experiment 1. In terms of visual acuity of the volunteers in the experimental group, for example, the test results of the visual acuity meter showed that their vision improved, their color discrimination was more delicate, and their ability to perceive low-contrast objects was enhanced. 2. Auditory acuity has been improved, lower frequencies or weaker sounds can be heard, and the source of the sound can be located more accurately. 3. The reaction speed was significantly accelerated, the visual response time and the auditory response time were shortened, and the performance was better in various reaction speed test tasks. ##6. Experimental risk assessment and response measures 1. **Risks of gene editing technology** -**Off-target effect**: It may lead to accidental editing of non-target genes and affect other normal physiological functions. Response measures: conduct a comprehensive bioinformatics analysis before gene editing to optimize the design of editing targets; conduct a full bioinformatics analysis after editing.